Pacific blue fluorophore.
FITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate - has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes. However, FITC is highly sensitive to pH changes and photo bleaching. In addition FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Yellow-Green 561 nm. Red 633 nm. View our complete Brightness Index. Our technical service team is available to help with any questions you may have on multicolor flow cytometry. Technical Service. Phone Toll-Free (US & Canada)1-877-273-3103. Phone (International): 1-858-768-5801. Email: Click Here.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... To add one or more spectra to the graph, click either "fluorophore" in the "Add" submenu on the left or click the center of the empty display panel - a menu will appear at the bottom of the page. To view a known dye, scroll down the menu on the left and click to select. The choice currently selected will be highlighted in blue.Pacific Orange Dye Cascade Blue and Other Pyrene Derivatives Cascade Yellow and Other Pyridyloxazole Derivatives Naphthalenes, Including Dansyl Chloride Dapoxyl Dye Bimane Data Table Ordering Information Related Tables Molecular Probes amine-reactive dyes—Table 1.1 Active esters and kits for labeling proteins and nucleic acids—Table 1.2
441. (in buffer): 1. (in antifade): 2. microscopy, flow cytometry, fluorescence microplate reader. Invitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability, and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets. In vivo, is an accessory photosynthetic pigment found in blue-green algae: APC-Cy7: Allophycocyanin – Cy7: Tandem conjugate where APC is coupled to a cyanine dye (Cy7) Longest wavelength emission in the family of fluorophores excited by the red laser: A488: Alexa Fluor 488: Incredibly stable, so very popular dye: Spectrum almost identical to ...
Blue BD Horizon V450 Pacific Blue™ BD Influx™ cell sorter Solid State 488 Green FITC Alexa Fluor® 488 Yellow PE Orange PE-Texas Red® Red PE-Cy5 PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE Orange PE-Texas Red® Red PE-Cy5 Infrared PE-Cy7 Solid State 640 Red APC Alexa Fluor® 647 Far Red Alexa Fluor®700 Infrared BD APC-H7 ... eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ...
We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of ...An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, (E20G/W37T)LplA, that ligates …Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due ...Pacific Blue™ 410: 455: 450/50: 1: The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and ...
The BFP filter combination is recommended when studying the following fluorophores: blue fluorescent protein, enhanced blue fluorescent protein, super glow blue fluorescent protein, Cascade Blue, catecholamine, fluorescamine, hydroxycoumarin, Leucophor SF, Pacific Blue, and TNS. The images presented in Figure 2 demonstrate the performance of ...
NovaFluor Blue 660-120S. Phiton technology. 509/614. 3. 660/20. Can be used to replace PE-tandem dyes with much less cross-excitation and lower spectral spillover, allowing detection of an additional marker labeled with NovaFluor Yellow 660 dye; NovaFluor Blue 660-120S is brighter than NovaFluor 660-40S.
Pacific Blue ; Pacific Green ; Pacific Orange ; Tetramethylrhodamine (TRITC) Texas Red (and Texas Red-X) PE, APC, related tandems APC-eFluor 780 ... Alexa Fluor 700 dye is a near-IR fluorophore used primarily for flow cytometry, optimally excited by the red laser. Photostability in buffer : Dim: 633-640: 720/30: 702: 723 : Relative staining ...Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression. Although its absorption peak is in deep blue, it is excited quite effectively in violet (Fig. 15.5), and before the appearance of the BV series fluorochromes, it was often used as a second “violet-driven” fluorochrome emitting to the right of Pacific Blue even though not particularly brilliant.Vio® Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy developed. by Miltenyi Biotec. VioBlue®, VioGreen™, Vio Bright FITC, PE-Vio® 615, PE-Vio 770, PerCP-Vio 700, Vio 515, Vio Bright 515, APC-Vio 770 are characterized by high fluorescence intensities and low spillover, making them an ideal choice for ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.
Pacific Orange dye is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 400/551 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression.Pacific Blue- eFluor450-vioBlue BV480 efluor506 BV510-vioGreen BV510 BV570 SB 600-BV605 Qdot605 SB 645-BV650 ... Fluorophore Fluorophore Fluorophore BB515 APC BV421After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation.
Vio Dye Fluorophores. Vio® and Vio® Bright Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy. They are characterized by high fluorescence intensities and low spillover, making them an ideal choice for multicolor applications. Combined with traditional fluorochromes, such as FITC, PE, PerCP, and APC, they ...
See full list on thermofisher.com The BFP filter combination is recommended when studying the following fluorophores: blue fluorescent protein, enhanced blue fluorescent protein, super glow blue fluorescent protein, Cascade Blue, catecholamine, fluorescamine, hydroxycoumarin, Leucophor SF, Pacific Blue, and TNS. The images presented in Figure 2 demonstrate the performance of ...Dory’s character in “Finding Nemo” is a blue tang fish. Blue tang fish are characterized by their bright blue coloring and yellow tail. These fish are found in the Pacific and Indian Oceans.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. …An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...* The excitation laser line represents commonly used lasers that excite the fluorochrome. It does not necessarily reflect the lasers available for each particular instrument. † APC and PE-Cy5 may be used together on instruments with cross-beam compensation. ... Pacific Blue ™ Blue 401 405, 407 ...
Ghost Dye™ Blue 516. Ghost Dye™ Red 710. Ghost Dye™ Red 780. Ghost Dye™ UV 450 ... Pacific Blue. Pacific Orange. PE. PE-Alexa Fluor® 610. PE-Alexa Fluor® 700. PE ...
Pacific Blue ; Pacific Green ; Pacific Orange ; Tetramethylrhodamine (TRITC) Texas Red (and Texas Red-X) PE, APC, related tandems APC-eFluor 780 ... Alexa Fluor 700 dye is a near-IR fluorophore used primarily for flow cytometry, optimally excited by the red laser. Photostability in buffer : Dim: 633-640: 720/30: 702: 723 : Relative staining ...
Our Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ...Live/Dead Blue or DAPI excited off the UV laser. For cell surface staining, BV421™ is significantly brighter than other spectrally equivalent fluorophores including Pacific Blue™, BD Horizon™ V450, eFluor® 450, and Alexa Fluor® 405. It has an extinction coefficient of 2,500,000 M-1cm-1 at 405 nm, and an aqueous solution quantumFluorochrome Chart. Masterful Panel Design Reveals Insight ... Pacific Blue DAPI FXCycle Violet Vybrant DyeCycle Violet 530/30 VL2 Pacific Green Horizon BV510 vGFP LIVE/DEAD Fixable Aqua 586/20 VL3 Horizon BV570 Qdot 605 LIVE/DEAD Fixable Yellow 615/20 VL4 Horizon BV605 Qdot 625Super Bright 600 Dye. Invitrogen eBioscience Super Bright 600 dye is tandem polymer dye, consisting of violet (405 nm) laser–excitable Super Bright 436 and an acceptor dye that emits fluorescence signal at 600 nm. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell ...An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.Dec 19, 2016 · We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of ... Oceania is a vast and beautiful region in the Pacific, comprising of thousands of islands scattered across the ocean. It is a diverse and culturally rich region that has attracted travelers from all over the world. If you are planning to ex...Pacific Blue: 455: 500–600: Pacific Green: 500 : Horizon V500, Brilliant Violet 510, VioGreen, Brilliant Violet 570: eFluor 506: 510: Pacific Orange: 550: 600–700: Super Bright 600: 600 : Brilliant Violet 605, ... Fluorophore staining index …Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. …
Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... See the characteristics of Pacific Blue dye and the range of antibody conjugates, kits, and other products related to this fluorophore.S2 Determination of the Kd of 16 for SA by Fluorescence Polarization (FP): The change in polarization of 16 was calculated by subtracting the average (n=3) polarization of the free ligand (P f), ...Instagram:https://instagram. watkins medical centrem. ed titlerotc for nursingbs community health When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. ... Pacific Blue, and BD Horizon V450 cannot ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. map of counties in kansaskansas state cheer Fluorophore Characteristics. JIR offers a wide range of fluorescent conjugates, covering the most commonly used excitation sources and filter sets from blue to infrared emissions. The following information describes some of the characteristics of fluorescent dye families and where they are best employed. Information about individual dyes can be ...Filter. Mirror. Some common fluorochromes. A. V525. 525/50. 495LP. BV510, Pacific Orange, Zombie Aqua. B. V450. 450/50. —. DAPI, BV421, AF405, Pacific Blue, ... micholee polsak S2 Determination of the Kd of 16 for SA by Fluorescence Polarization (FP): The change in polarization of 16 was calculated by subtracting the average (n=3) polarization of the free ligand (P f), ...Pacific Blue, or systemically called 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its maximum excitation wavelength is 401 nm and its maximum emission wavelength is 452 nm. Even at a pH of about 7, its fluorescence is still very high. Pacific Blue is a member of the Pacific Dye Group.