Ku protein.

Ku is a dimeric protein complex that binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Ku is evolutionarily conserved from bacteria to humans. The ancestral bacterial Ku is a homodimer (two copies of the same protein bound to each other). [2]Here, a 3D structural model of the full-length Ku protein is proposed on the basis of single-particle EM experiments. Results. Ku purification and its interaction with DNA. Ku70-Ku80 was purified from HeLa cells (Fig 1F). The ability of our preparation to form a stable complex with DNA was tested by incubation with a 54 bp oligonucleotide.CA125 is a protein that is found in blood. A small sample of blood will be taken from your arm and sent to a lab where a simple test will measure the level of CA125 in your blood sample. In most healthy women the level of CA125 is usually less than 35. However, some women do have a naturally high level of CA125 in their blood.PhD Novel barrier layers for packaging materials suitable for protein-rich foods (ref. BAP-2023-206): We offer a PhD position at the research group of Meat Technology and Science of Protein-Rich Foods (MTSP) at the Ku Leuven, Campus Gent.Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified by researchers as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis, and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA ...

Aravind L, Koonin EV . 2001 Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system ...

Immunoaffinity-purified Ku protein was used to screen sera from patients with systemic lupus erythematosus (SLE), scleroderma, myositis and Sjögren's syndrome for anti-Ku antibodies in a quantitative immunoblot assay. Sixteen percent of the 159 studied sera were reactive with the Ku protein; signifi …The gene product defective in radiosensitive CHO mutants belonging to ionizing radiation complementation group 5, which includes the extensively studied xrs mutants, has recently been identified as Ku80, a subunit of the Ku protein and a component of DNA-dependent protein kinase (DNA-PK). Several gr …

The current thinking is that Ku protein binds to DNA discontinuously and in a sequence independent manner, carrying out a DNA-protective role . Once bound to DNA, Ku proteins recruit and activate the catalytic DNAPKcs subunit which can phosphorylate Ku and other neighboring DNA-bound proteins . It has also been reported that DNAPKcs self ...DNA double-strand breaks (DSBs) are considered most hazardous for the organism and are generally repaired by nonhomologous end joining (NHEJ), where Ku protein acts as the sensor and binds to specific protein kinases, leading to the joining of the broken strands [120].Ku protein, a heterodimer of 70 and 83 kDa polypeptides, is the regulatory component of the DNA-dependent protein kinase (DNA-PK). Ku protein binds to DNA ends and is essential for DNA double-strand break repair and V(D)J recombination. Although there is some evidence that Ku protein also binds RNA, …Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded …Ku protein is a complex of two subunits, Ku70 and Ku80. Ku is suspected to participate in both DNA double-strand break repair and transcription. Since both of these processes take place in the cell nucleus, we have been investigating the subcellular localization and nuclear transport of Ku proteins. …

The observed effects cannot be accounted for by the differential expression levels of Ku protein in control and SC4 aptamer-expressing cells, but are rather due to augmented DNA binding-capacity of Ku protein, as demonstrated in in vitro studies. Thus, RNA aptamers against Ku protein show potential to sensitize MCF-7 breast carcinoma cells to ...

Apr 1, 2020 · The Ku protein is a highly abundant protein in mammalian cells made up by two subunits, Ku70 and Ku80 [1]. The most well-known Ku function is participation in the repair of DNA double-strand breaks (DSBs) through the non-homologous end joining (NHEJ) pathway where the Ku heterodimer acts as the main sensor of DSBs.

The Ku protein, a heterodimer of Ku70 and Ku80, binds to chromosomal replication origins maximally at G1-phase and plays an essential role in assembly of origin recognition complex. However, the mechanism regulating such a critical periodic activity of Ku remained unknown. Here, we establish human Ku70 as a novel target of cyclin B1-Cdk1, which ...Ku adopts ring shaped structure and acts as a DNA targeting subunit of the DNA-dependent protein kinase catalytic subunit (DNA PKcs or XRCC7) which along with Ku forms DNA-PK holoenzyme. DNA-PKcs induce an inward translocation of Ku protein allowing DNA-PKcs to contact opposing DSB ends.Ku is an abundant, highly conserved DNA binding protein found in both prokaryotes and eukaryotes that plays essential roles in the maintenance of genome integrity. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the "classical" non-homologous end joining (C-NHEJ) pathway ...Here, the double-strand breaks are primarily detected by Ku protein (Ku70-Ku80 heterodimer) [117], which then recruits the DNA protein kinase catalytic subunits (DNA-PKcs), forming Ku-DNA-PK complex surrounding the broken strands. When a double-strand break occurs, the configuration at the broken ends can vary. They can have blunt ends, 5 ...Ku binds broken DNA ends with strong affinity and recruits DNA-dependent protein kinase catalytic subunit, leading to recruitment of Artemis, XRCC4, DNA ligase IV, and XRCC4-like factor to ...These studies clearly demonstrate that the absence of Ku protein results in radiation hypersensitivity, proportional dwarfism, deficiencies in DNA DSB repair and V(D)J recombination, and impaired lymphocyte development. The use of the Ku- and DNA-PKcs-deficient cell lines has yielded much information on the roles of Ku and DNA-PKcs in ...

Here, small angle X-ray scattering (SAXS) and mutational analyses show APLF is largely an intrinsically disordered protein that binds Ku, Ku/DNA-PKcsmore » X4L4 assembles with Ku heterodimers linked to DNA-PKcs via flexible Ku80 C-terminal regions (Ku80CTR) in a complex stabilized through APLF interactions with Ku, DNA-PK, and X4L4.Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without ...Ku protein, together with Rap1p, a telomeric DNA (TG 1-3 repeat)-binding protein, is also required to initiate transcriptional silencing, or telomere-position effect (TPE). Here, we provide evidence for a direct role of Ku in TPE, which is most likely to be in either the recruitment or activation of Sir4 protein at the telomere. Surprisingly ...Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system .Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system . The Ku protein or X-ray repair cross-complementing protein binds to the ends of double-strand breaks and it is required in DNA-repair for non-homologous end joining. …KU80 was one of the first radiosensitivity genes discovered from such studies (Taccioli et al., 1994 ). Its function is to protect the ends of DNA from further degradation and, importantly, to recruit the protein kinase DNA-PKcs (DNA-dependent protein kinase catalytic subunit (officially known as PRKDC)).

We have characterized the biochemical nature of the Ku protein, the antigen recognized by autoantibodies from certain patients with scleroderma-polymyositis overlap syndrome. From extracts of HeLa cells labeled with [32P]orthophosphate, anti-Ku antibodies precipitated a high molecular weight nucleic acid identified as DNA because of sensitivity ...These results suggest that Ku proteins are specific to breast cancer progression and indicate that in intestinal cancer cells, tumoregenesis is not associated with Ku protein expression. Many studies have suggested a key role of Ku in cancer development and progression, acting as either tumor suppressor gene or as oncogene 45 .

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V (D)J recombination. The mechanism underlying the ... pfam08785. Location:558 → 672.Ku possesses ATPase and DNA helicase activity and is the DNA-binding moiety of the high-molecular-weight DNA-dependent protein kinase (DNA-PK) in mammals, which phosphorylates DNA repair factors, p53, and other proteins (S mith and J ackson 1999).damage or co-precipitate Ku protein complexes if over-expressed. by itself as a GFP-fusion protein (Figs 2a and 3a; 'WRN-nA'). Mutation of the N-terminal KBM reduced Ku co-precipitation by.The prokaryotic Ku protein is a homodimer of approximately 30–40 kDa, consisting of a core domain conserved with the eukaryotic Ku homolog, and a C-terminus unique to bacteria (McGovern et al., 2016). The C-terminus of Ku can be further subdivided into a minimal and an extended region. Oct 14, 2023 · the Ku protein, which is present in many bacteria such as Actinomycetes, Bacillus, Agrobacterium and Methanosar-cina [7–13], generally binds to DSBs but does not inter-act with closed circular DNA because it first recognizes DNA ends and then transfers them to internal sites [14]. The LigD protein, an ATP-dependent ligase also found inJul 10, 2023 · Several years later, a team co-led by Enrico Cappellini, a protein chemist at the University of Copenhagen (KU), sequenced tooth proteins from roughly 800,000-year-old remains belonging to a ...Two distinct and complementary pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), are employed by mammalian cells to repair DNA DSBs. Each pathway is tightly controlled in response to increased DSBs. The Ku heterodimer has been shown to play a regulatory role in NHEJ repair. Ku80 ubiquitination contributes to the ...Workflow of the HT protein production and characterization pipeline. It enables: (1) easy selection of ORFs encoding target proteins from any existing Gateway clone libraries; (2) rapid and ...

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Anti-Ku antibodies are reported in various connective tissue diseases and the Ku complex can be responsible for a very strong autoimmune answer in autoimmune disease. Nowadays, anti-Ku antibodies are detected by ELISA, counterimmunoelectrophoresis (CIE), immunoblot (IB) and new highly performant tec …

DNA-dependent protein kinase (DNA-PK) is a large protein complex central to the nonhomologous end joining (NHEJ) DNA-repair pathway. It comprises the DNA-PK catalytic subunit (DNA-PKcs) and the heterodimer of DNA-binding proteins Ku70 and Ku80. Here, we report the cryo-electron microscopy (cryo-EM) …Critical to NHEJ is the DNA-dependent interaction of the Ku70/80 heterodimer with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to form the DNA-PK holoenzyme. However, precisely how Ku recruits DNA-PKcs to DSBs ends to enhance its kinase activity has remained enigmatic, with contradictory findings reported in the literature.Ku, another protein required for NHEJ, also stimulates LX ligation non-specifically and enhances DNA bridging [17]. Indeed, there is a striking similarity between Ku and AHNAK in this context since Ku also interacts with LX on the DNA molecule and, based on a specific interaction with LX, has been suggested to recruit LX to DNA [53] .The DNA-binding protein Ku plays a critical role in a variety of cellular processes, including the repair of double-stranded DNA breaks and V (D)J recombination. Paradoxically, while Ku is required for double-stranded break repair by non-homologous end-joining, in many organisms, Ku is also associated with telomeres.High blood protein is not usually accompanied by symptoms and is usually diagnosed through a blood test, according to Mayo Clinic. While it is not a disorder, high blood protein can sometimes indicate the presence of a disease.Here, a 3D structural model of the full-length Ku protein is proposed on the basis of single-particle EM experiments. Results Ku purification and its interaction with DNA. Ku70-Ku80 was purified from HeLa cells . The ability of our preparation to form a stable complex with DNA was tested by incubation with a 54 bp oligonucleotide.While Ku in eukaryotes is often a multi-domain protein functioning in DNA repair of physiological and pathological DNA double-stranded breaks, Ku in prokaryotes is a single-domain protein ...The inability to rescue activity with purified Ku suggests either that the role of Ku is indirect or that the endogenous Ku differs in some way from the exogenous protein. Interestingly, a different CHO cell mutant that lacks the DNA-dependent protein kinase catalytic subunit, a protein in the same repair pathway as Ku, also exhibits a decrease ...Workflow of the HT protein production and characterization pipeline. It enables: (1) easy selection of ORFs encoding target proteins from any existing Gateway clone libraries; (2) rapid and ...The BJS_8160 was identified as a non-homologous end-joining protein Ku (Ku protein); these proteins are specialized in repairing DNA damages In S. meliloti Ku proteins have been identified in both free-living conditions and bacteroids , and related to stress conditions, as heat and nutrient starvation ; however, in S. meliloti, Ku protein was ...

Therefore, Ku protein neddylation is associated with Ku protein ubiquitination, which may induce apoptotic cell death. Astaxanthin, an oxygen-containing carotenoid (xanthophyll), is present in marine algae, red yeast, fungi, and various plant and animal sources. It is a fat-soluble nutrient2.1. Formation of DNA-protein complexes by yKu and Dnl4/Lif1 on oligonucleotide substrates. Previously, Dynan and colleagues used photocrosslinking to determine the relative positions of Ku and DNA PKcs in DNA-proteins complexes formed on a DNA end [18, 19].Notably, these studies revealed that Ku was displaced inward along the DNA duplex as a consequence of DNA PKcs binding.The Ku antigen has been identified as a non-histone DNA-binding protein complex composed of two polypeptides: 86 kDa and 70 kDa. Initial competition experiments with four monoclonal antibodies specific for the 86 kDa subunit of Ku protein suggested there was more than one epitope on this polypeptide. To determine the region for these epitopes ...Instagram:https://instagram. what does colorguard doespn illini footballpress coferenceku vs kansas state football Ku protein degradation. In conclusion, dietary astaxanthin supplementation or astaxanthin-rich food consumption may be effective for preventing or delaying oxidative stress-mediated cell dam-age by suppressing Ku protein loss and apoptosis in gastric epithelial cells. brain greenfactory near me The dissection of the Ku protein from eukaryotes and prokaryotes into individual domains described above suggests an evolutionary scenario for these proteins. The Ku core is an ancient domain that was probably present in bacteria and archea even before the advent of the eukaryotes. There are clear indications that, in these organisms, the Ku ...If peanut IgE is 0.10 kU/L or greater, then 7 peanut components (Ara h 2, Ara h 1, Ara h 3, Ara h 6, Ara h 8, Ara h 9, and profilin Bet v2) are performed at an additional charge. ... Group 1 Fagales-related protein (h 8) PR-10 Protein (h 8) Non-specific Lipid Transfer Protein (ns-LTP) (h 9) PR-14 Protein (h 9) Bet v2. Profilin. audacity platform Ku protein complex, formed by Ku70 and Ku80, is necessary during non-homologous end joining (NHEJ)-mediated DNA repair, in order to prevent extensive DNA damage. Mice deficient in Ku70, Ku80 or both exhibit early aging phenotypes and have a lifespan of ~37 weeks [ 104 ].Benzonase® Nuclease HC, Purity >99% 25 KU 71206-3 Benzonase® Nuclease, Purity >90% 2.5 KU 10 KU 70746-4 70746-3 Benzonase® Nuclease HC, Purity >90% 25 KU 71205-3 Ideal for a variety of applications ... Protein Extraction Reagent and the other half was lysed with BugBuster plus Benzonase® Nuclease following the recommend protocol.NHEJ requires a protein complex consisting of the heterodimer of Ku70 and Ku80 (Ku complex) that recognizes DSB free ends and the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) (1, 5, 6) that recruits core NHEJ factors including Artemis, XRCC4, Ligase IV, and XLF on DSB break sites ().